Biomarkers Library

Understand your numbers before you build your protocol.

Key biomarkers explained in plain language, what they measure, what optimal looks like, and why they matter before any peptide research. Test first. Always.

15 Biomarkers
6 Body Systems
Optimal Ranges Listed
No Account Required

Your labs are your starting point, not an afterthought.

Know Your Baseline

Without baseline labs you have no way to know what's changing, what's working, or what needs attention. Numbers give your research context it can't have without them.

Catch Contraindications

Certain biomarker patterns make specific peptide research inappropriate or higher risk. Labs reveal what symptoms can't, and often point to a different root cause entirely.

Track Progress

Repeat labs at 8–12 week intervals give you objective data instead of guesswork. Without before-and-after measurements, you have no way to evaluate what your research is doing.

Showing 19 of 19 biomarkers
Growth Hormone 🔴 Essential

IGF-1

Insulin-like Growth Factor-1

Insulin-like growth factor 1, a growth-axis marker involved in growth-hormone signaling, reviewed alongside metabolic and hormone markers. Educational context only.

Related goals

Longevity Muscle Recovery Hormonal Balance Fat Loss
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Hormonal 🔴 Essential

Testosterone

Testosterone Biomarkers (Overview)

Overview of the testosterone-related markers — total testosterone, free testosterone, SHBG, LH, FSH, and DHEA-S — and how they are reviewed together. Educational context only.

Related biomarkers

Total Testosterone Free Testosterone SHBG LH and FSH
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Hormonal 🔴 Essential

Total Testosterone

Total Testosterone (bound + unbound)

A commonly measured hormone marker: the combined amount of testosterone circulating in the blood, reviewed alongside free testosterone and SHBG. Educational context only.

Related biomarkers

Free Testosterone SHBG LH and FSH DHEA-S
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Hormonal 🔴 Essential

Free Testosterone

Free Testosterone (unbound fraction)

The unbound fraction of testosterone not attached to SHBG or albumin, commonly reviewed alongside total testosterone and SHBG. Method-dependent. Educational context only.

Related biomarkers

Total Testosterone SHBG LH and FSH DHEA-S
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Stress & Adrenal 🔴 Essential

Cortisol

AM Serum Cortisol

A steroid hormone produced by the adrenal glands that follows a daily rhythm, commonly reviewed in adrenal-related and hormone-related lab panels. Educational context only.

Related goals

Sleep & Stress Hormonal Balance Longevity
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Inflammation 🔴 Essential

hsCRP

High-Sensitivity C-Reactive Protein

High-sensitivity C-reactive protein, an inflammation-related marker made by the liver, reviewed alongside metabolic and lipid markers. Educational context only.

Related goals

Gut Health Immune Support Muscle Recovery Fat Loss
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Inflammation 🟡 Important

Ferritin

Iron Storage Protein

"The body's iron-storage protein and the standard marker of iron reserves, but it also rises with inflammation."

Optimal range Men 100–300 · Women 50–150 ng/mL

Related goals

Skin & Regeneration Hormonal Balance
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Metabolic 🔴 Essential

Fasting Insulin

Fasting Serum Insulin

Insulin measured after a fasting period, a glucose-related marker that provides insulin-response context alongside fasting glucose and HbA1c. Educational context only.

Related goals

Fat Loss Metabolic Health Longevity
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Metabolic 🔴 Essential

Fasting Glucose

Fasting Blood Glucose

A point-in-time glucose-related marker measured after a fasting period, commonly reviewed alongside HbA1c and fasting insulin. Educational context only.

Related goals

Fat Loss Metabolic Health
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Metabolic 🔴 Essential

HbA1c

Glycated Hemoglobin

Glycated hemoglobin, a longer-term glucose-related marker that reflects average glucose exposure over the life of red blood cells. Educational context only.

Related goals

Fat Loss Metabolic Health Longevity
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Metabolic 🟡 Important

Liver Enzymes

ALT, AST, GGT

"A standard blood panel that offers a window into liver health, often reviewed as a baseline in metabolic research."

Optimal range ALT/AST 7–35 U/L (functional)

Related goals

Metabolic Health Longevity
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Immune & Hormonal 🔴 Essential

Vitamin D

25-OH Vitamin D

"Often called the sunshine vitamin, but it works more like a hormone, affecting immune function, mood, testosterone, and bone health all at once."

Optimal range 50–80 ng/mL (optimal) · 30–80 ng/mL (standard normal)

Related goals

Immune Support Longevity Hormonal Balance Skin & Regeneration
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Thyroid 🔴 Essential

TSH

Thyroid Stimulating Hormone

"The signal your brain sends to your thyroid, high TSH means the thyroid isn't responding enough, low TSH means it may be overactive."

Optimal range 0.8–2.0 mIU/L (optimal) · 0.4–4.0 mIU/L (standard normal)

Related goals

Longevity Sleep & Stress Hormonal Balance Cognitive Performance
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Hormonal 🟡 Important

SHBG

Sex Hormone Binding Globulin

Sex hormone-binding globulin, the hormone-binding protein made mainly by the liver that binds testosterone and estradiol and adds context alongside total and free testosterone. Educational context only.

Related goals

Hormonal Balance Sexual Health Fat Loss
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Hormonal 🟡 Important

LH & FSH

Luteinizing Hormone & Follicle Stimulating Hormone

Pituitary hormones that provide pituitary-gonadal signaling context, commonly reviewed alongside testosterone, SHBG, and free testosterone. Educational context only.

Related goals

Hormonal Balance Sexual Health
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Hormonal 🟡 Important

Estradiol

Estradiol (E2)

Estradiol (E2), a major form of estrogen present in all sexes, commonly reviewed alongside testosterone, SHBG, and other sex hormone markers. Educational context only.

Related goals

Hormonal Balance Sexual Health Longevity
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Cardiovascular 🟡 Important

ApoB

Apolipoprotein B

Apolipoprotein B, a lipid-related marker that provides a particle-number-related view of atherogenic lipoproteins, reviewed alongside lipid and metabolic markers. Educational context only.

Related goals

Fat Loss Longevity Metabolic Health
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Longevity & Adrenal 🔵 Advanced

DHEA-S

Dehydroepiandrosterone Sulfate

Dehydroepiandrosterone sulfate, an adrenal androgen-related marker commonly reviewed alongside testosterone, SHBG, and other hormone markers. Educational context only.

Related goals

Longevity Hormonal Balance Cognitive Performance
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Longevity & Cardiovascular 🔵 Advanced

Homocysteine

Plasma Homocysteine

"An amino acid byproduct that builds up when methylation pathways aren't working well, elevated levels are linked to cardiovascular and cognitive risk."

Optimal range Under 8 µmol/L (optimal) · Under 15 µmol/L (standard normal)

Related goals

Longevity Cognitive Performance Cardiovascular Health
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Which biomarkers should you test first?

Three tiers of testing, ordered by what's needed before each stage of research. Work through them in sequence with a licensed healthcare provider.

Tier 1 : Essential Baseline

Test these before any peptide research

hsCRP · Fasting Glucose · Fasting Insulin · HbA1c · TSH · Vitamin D · Comprehensive Metabolic Panel · CBC with diff

"This panel gives you the metabolic, inflammatory, and thyroid context needed for any peptide category."

Tier 2 : GH Protocol Ready

Add these before GH secretagogue research

IGF-1 · Total & Free Testosterone · SHBG · LH & FSH · Estradiol · AM Cortisol · Free T3 · Free T4 · Anti-TPO

"Required before Ipamorelin, CJC-1295, Sermorelin, or any GH secretagogue research."

Tier 3 : Advanced Longevity Panel

Add these for comprehensive optimization

ApoB · DHEA-S · Homocysteine · Ferritin · Omega-3 Index · Uric Acid · NAD+ levels

"For those working with a longevity or functional medicine clinician on a multi-peptide protocol."

Ready to understand your results?

The Lab Testing Guide explains exactly how to prepare, what to order, and how to read your results in the context of peptide research.

For educational and research purposes only. Not medical advice. Always consult a licensed healthcare provider before making any health decisions.